 Mars Hill UniversityDIIMars Hill, NC · Lions | South Atlantic Conference | Griffin StechmillerHead coach since at least 2023 | 36 | |
 Maryville UniversityDIISt. Louis, MO · Saints | Great Lakes Valley Conference | Scott StarkHead coach since at least 2023 | 52 | |
 Mercy UniversityDIIDobbs Ferry, NY · Mavericks | East Coast Conference | Ryan MacSpadyen5th season since 2023 | 41 | |
 Molloy UniversityDIIRockville Centre, NY · Lions | East Coast Conference | William Dunn6th season since 2022 | 43 | |
 Newberry CollegeDIINewberry, SC · Wolves | South Atlantic Conference | Nick Cotter | 59 | |
 North Greenville UniversityDIITigerville, SC · Trailblazers | Conference Carolinas | Corey StrussHead coach since at least 2023 | 60 | |
 Northwest Nazarene UniversityDIINampa, ID · Nighthawks | Rocky Mountain Athletic Conference | Tom BlanchardHead coach since at least 2024 | 39 | |
 Northwood UniversityDIIMidland, MI · Timberwolves | Great Midwest Athletic Conference | Jacob Fredal4th season since 2024 | 41 | |
 Pace UniversityDIIPleasantville, NY · Setters | Northeast 10 Conference | Tom Mariano | 52 | |
 Palm Beach Atlantic UniversityDIIWest Palm Beach, FL · Sailfish | Sunshine State Conference | Daniel Carson2nd season since 2026 | 49 | |
 Point Park UniversityDIIPittsburgh, PA · Pioneers | Great Midwest Athletic Conference | Cody GolonHead coach since at least 2026 | 45 | |
 Post UniversityDIIWaterbury, CT · Eagles | Central Atlantic Collegiate Conference | Patrick Yannarelli4th season since 2024 | 27 | |
 Quincy UniversityDIIQuincy, IL · Hawks | Great Lakes Valley Conference | Jacob Acevedo3rd season since 2025 | 31 | |
 Roberts Wesleyan UniversityDIIRochester, NY · Redhawks | East Coast Conference | Rocky DelfinoHead coach since at least 2023 | 33 | |
 Rockhurst UniversityDIIKansas City, MO · Hawks | Great Lakes Valley Conference | Kevin Kelley15th season since 2013 | 60 | |
 Rollins CollegeDIIWinter Park, FL · Tars | Sunshine State Conference | Christopher Lewis12th season since 2016 | 52 | |
 Saint Anselm CollegeDIIManchester, NH · Hawks | Northeast 10 Conference | Drew Bourdeau3rd season since 2025 | 49 | |
 Saint Leo UniversityDIISt. Leo, FL · Lions | Sunshine State Conference | Brad Jorgensen22nd season since 2006 | 39 | |
 Saint Michael's CollegeDIIColchester, VT · Purple Knights | Northeast 10 Conference | Alex SmithHead coach since at least 2023 | 53 | |
 Seton Hill UniversityDIIGreensburg, PA · Griffins | Great Midwest Athletic Conference | Brian NovotnyHead coach since at least 2023 | 56 | |
 Shorter UniversityDIIRome, GA · Hawks, Lady Hawks | Conference Carolinas | Jordan CoatesHead coach since at least 2026 | 22 | |
 Southern New Hampshire UniversityDIIManchester, NH · Penmen | Northeast 10 Conference | Paul Calkins38th season since 1990 | 56 | |
 St. Thomas Aquinas CollegeDIISparkill, NY · Spartans | East Coast Conference | Not listed | 32 | |
 Thomas More UniversityDIICrestview Hills, KY · Saints | Great Midwest Athletic Conference | Sean McGinnis5th season since 2023 | 29 | |
 Tiffin UniversityDIITiffin, OH · Dragons | Great Midwest Athletic Conference | Travis Brown5th season since 2023 | 52 | |
 Tusculum UniversityDIIGreeneville, TN · Pioneers | South Atlantic Conference | Mike BrisbinHead coach since at least 2024 | 42 | |
 University of Alabama in HuntsvilleDIIHuntsville, AL · Chargers | Peach Belt Conference | Jordan Warren3rd season since 2025 | 67 | |
 University of BridgeportDIIBridgeport, CT · Purple Knights | Central Atlantic Collegiate Conference | Ryan McAleaveyHead coach since at least 2023 | 20 | |
 University of IndianapolisDIIIndianapolis, IN · Greyhounds | Great Lakes Valley Conference | Greg StocksHead coach since at least 2023 | 54 | |
 University of MontevalloDIIMontevallo, AL · Falcons | Peach Belt Conference | Jason Lange10th season since 2018 | 65 | |
 University of Mount OliveDIIMount Olive, NC · Trojans | Conference Carolinas | Dylan Price13th season since 2015 | 46 | |
 University of TampaDIITampa, FL · Spartans | Sunshine State Conference | J.B. ClarkeHead coach since at least 2024 | 63 | |
| University of the District of Columbia DIIWashington, DC · Firebirds | East Coast Conference | Matthew Marrett | 20 | |
 Virginia State UniversityDIIPetersburg, VA · Trojans | Peach Belt Conference | Shaun ChurchHead coach since at least 2024 | 33 | |
 Walsh UniversityDIINorth Canton, OH · Cavaliers | Great Midwest Athletic Conference | Kyle DeCrispino1st season since 2027 | 48 | |
 Westminster UniversityDIISalt Lake City, UT · Griffins | Rocky Mountain Athletic Conference | Brad Lavoie2nd season since 2026 | 60 | |
 Wheeling UniversityDIIWheeling, WV · Cardinals | Great Midwest Athletic Conference | Gage Bradshaw7th season since 2021 | 32 | |
 William Jewell CollegeDIILiberty, MO · Cardinals | Great Lakes Valley Conference | Andrew Briones1st season since 2027 | 44 | |
 Wilmington UniversityDIINew Castle, DE · Wildcats | Central Atlantic Collegiate Conference | Christian ZwickertHead coach since at least 2023 | 40 | |
 Wingate UniversityDIIWingate, NC · Bulldogs | South Atlantic Conference | Timothy Boyle11th season since 2017 | 56 | |
