 Tuskegee UniversityDIITuskegee, AL · Tigers | Independent | Not listed | 37 | |
 Union UniversityDIIJackson, TN · Bulldogs | Gulf South Conference | Steven Cox9th season since 2018 | 29 | |
 University of Alabama in HuntsvilleDIIHuntsville, AL · Chargers | Gulf South Conference | John Carrier9th season since 2018 | 30 | |
 University of BridgeportDIIBridgeport, CT · Purple Knights | Central Atlantic Collegiate Conference | Peter Doneit | 33 | |
 University of California, Merced (UC Merced)DIIMerced, CA · Golden Bobcats | California Collegiate Athletic Association | Albert Martins | 41 | |
 University of CharlestonDIICharleston, WV · Golden Eagles | Mountain East Conference | Daniel Smee7th season since 2020 | 53 | |
 University of Colorado Colorado SpringsDIIColorado Springs, CO · Mountain Lions | Rocky Mountain Athletic Conference | Lewis Wilcox6th season since 2021 | 32 | |
 University of FindlayDIIFindlay, OH · Oilers | Great Midwest Athletic Conference | Andy Smyth38th season since 1989 | 45 | |
 University of Hawai'i at HiloDIIHilo, HI · Vulcans | Pacific West Conference | Diego Marroquin3rd season since 2024 | 32 | |
 University of Illinois at SpringfieldDIISpringfield, IL · Prairie Stars | Great Lakes Valley Conference | Graham Shaw3rd season since 2024 | 30 | |
 University of IndianapolisDIIIndianapolis, IN · Greyhounds | Great Lakes Valley Conference | Gabe Hall5th season since 2022 | 63 | |
 University of JamestownDIIJamestown, ND · Jimmies | Independent | Justin Gilfert1st season since 2026 | 40 | |
 University of Missouri-St. LouisDIISt. Louis, MO · Tritons | Great Lakes Valley Conference | Josh Alderson3rd season since 2024 | 27 | |
 University of MontevalloDIIMontevallo, AL · Falcons | Gulf South Conference | Bruce Dietterle13th season since 2014 | 34 | |
 University of Mount OliveDIIMount Olive, NC · Trojans | Conference Carolinas | Ian Spooner | 41 | |
 University of North GeorgiaDIIDahlonega, GA · Nighthawks | Peach Belt Conference | Anel Lilic4th season since 2023 | 31 | |
 University of Pittsburgh at JohnstownDIIJohnstown, PA · Mountain Cats | Pennsylvania State Athletic Conference | Eric Kinsey | 35 | |
| University of Puerto Rico at Bayamón DIIBayamón, PR · Cowboys | Independent | Not listed | — | No roster |
 University of South Carolina AikenDIIAiken, SC · Pacers | Peach Belt Conference | Nick Sakiewicz3rd season since 2024 | 31 | |
 University of TampaDIITampa, FL · Spartans | Sunshine State Conference | Adrian Bush | 34 | |
 University of Texas at DallasDIIRichardson, TX · Comets | Lone Star Conference | Jason Hirsch | 36 | |
 University of Texas at TylerDIITyler, TX · Patriots | Lone Star Conference | Kenny Jones | 35 | |
| University of the District of Columbia DIIWashington, DC · Firebirds | East Coast Conference | Matthew L. Thompson | 30 | |
 University of West AlabamaDIILivingston, AL · Tigers | Gulf South Conference | Ross Nelson | 26 | |
 University of Wisconsin-ParksideDIIKenosha, WI · Rangers | Great Lakes Intercollegiate Athletic Conference | Jason Zitzke16th season since 2011 | 34 | |
 Upper Iowa UniversityDIIFayette, IA · Peacocks | Great Lakes Valley Conference | Jeancy Matumona-Bau1st season since 2026 | 42 | |
 Vanguard UniversityDIICosta Mesa, CA · Lions | Pacific West Conference | Jesus (Chuy) Miramontes5th season since 2022 | 34 | |
 Virginia State UniversityDIIEttrick, VA · Trojans | Independent | Not listed | 29 | |
 Walsh UniversityDIINorth Canton, OH · Cavaliers | Great Midwest Athletic Conference | Andy Hoggarth | 50 | |
 West Chester UniversityDIIWest Chester, PA · Golden Rams | Pennsylvania State Athletic Conference | Michael Benn | 28 | |
 West Liberty UniversityDIIWest Liberty, WV · Hilltoppers | Mountain East Conference | Sean Regan8th season since 2019 | 35 | |
 West Texas A&M UniversityDIICanyon, TX · Buffaloes | Lone Star Conference | Butch Lauffer36th season since 1991 | 45 | |
 West Virginia Wesleyan CollegeDIIBuckhannon, WV · Bobcats | Mountain East Conference | Glenn Francis | 54 | |
 Western Oregon UniversityDIIMonmouth, OR · Wolves | Great Northwest Athletic Conference | Andrew May | 38 | |
 Western Washington UniversityDIIBellingham, WA · Vikings | Great Northwest Athletic Conference | Greg Brisbon14th season since 2013 | 23 | |
 Westminster UniversityDIISalt Lake City, UT · Griffins | Rocky Mountain Athletic Conference | Josh Pittman10th season since 2017 | 46 | |
 Westmont CollegeDIISanta Barbara, CA · Warriors | Pacific West Conference | Morgan Cathey | 30 | |
 Wheeling UniversityDIIWheeling, WV · Cardinals | Mountain East Conference | Jack Shearer1st season since 2026 | 29 | |
 William Jewell CollegeDIILiberty, MO · Cardinals | Great Lakes Valley Conference | Keith Buderus1st season since 2026 | 34 | |
 Wilmington UniversityDIINew Castle, DE · Wildcats | Central Atlantic Collegiate Conference | Nick Papanicolas | 31 | |
