 University of Alabama in HuntsvilleDIIHuntsville, AL · Chargers | Gulf South Conference | James Ballamy | 30 | |
 University of BridgeportDIIBridgeport, CT · Purple Knights | Central Atlantic Collegiate Conference | Tania Armellino | 35 | |
 University of California, Merced (UC Merced)DIIMerced, CA · Golden Bobcats | California Collegiate Athletic Association | Roman DuChateau | 42 | |
 University of Central MissouriDIIWarrensburg, MO · Jennies | Mid-America Intercollegiate Athletics Association | Lewis Theobald20th season since 2007 | 25 | |
 University of Central OklahomaDIIOklahoma, OK · Bronchos | Mid-America Intercollegiate Athletics Association | Amel Muhamedagic | 32 | |
 University of CharlestonDIICharleston, WV · Golden Eagles | Mountain East Conference | Sarah Leiby-Gill | 27 | |
 University of Colorado Colorado SpringsDIIColorado Springs, CO · Mountain Lions | Rocky Mountain Athletic Conference | J. B. Belzer | 27 | |
 University of FindlayDIIFindlay, OH · Oilers | Great Midwest Athletic Conference | Sierra Tom | 32 | |
 University of Hawai'i at HiloDIIHilo, HI · Vulcans | Pacific West Conference | Gene Okamura | 33 | |
 University of Illinois at SpringfieldDIISpringfield, IL · Prairie Stars | Great Lakes Valley Conference | Scott Myers | 32 | |
 University of IndianapolisDIIIndianapolis, IN · Greyhounds | Great Lakes Valley Conference | Nick Smith | 30 | |
 University of JamestownDIIJamestown, ND · Jimmies | Northern Sun Intercollegiate Conference | Nick Becker | 30 | |
 University of MaryDIIBismarck, ND · Marauders | Northern Sun Intercollegiate Conference | Sarah Cook | 28 | |
 University of Minnesota CrookstonDIICrookston, MN · Golden Eagles | Northern Sun Intercollegiate Conference | Danny Burstein | 24 | |
 University of Minnesota DuluthDIIDuluth, MN · Bulldogs | Northern Sun Intercollegiate Conference | Greg Cane33rd season since 1994 | 32 | |
 University of Missouri-St. LouisDIISt. Louis, MO · Tritons | Great Lakes Valley Conference | Stephen Cavallo | 29 | |
 University of MontevalloDIIMontevallo, AL · Falcons | Gulf South Conference | Trey Gregory | 29 | |
 University of Mount OliveDIIMount Olive, NC · Trojans | Conference Carolinas | Glad Bugariu | 33 | |
 University of Nebraska at KearneyDIIKearney, NE · Lopers | Mid-America Intercollegiate Athletics Association | Rob Breton | 32 | |
 University of North Carolina at PembrokeDIIPembroke, NC · Braves | Conference Carolinas | Lars Andersson26th season since 2001 | 25 | |
 University of North GeorgiaDIIDahlonega, GA · Nighthawks | Peach Belt Conference | Kayley Ralton | 28 | |
 University of Pittsburgh at JohnstownDIIJohnstown, PA · Mountain Cats | Pennsylvania State Athletic Conference | Aideen O'Donoghue | 29 | |
| University of Puerto Rico at Bayamón DIIBayamón, PR · Cowgirls | Independent | Not listed | — | No roster |
| University of Puerto Rico, Río Piedras DIISan Juan, PR · Jerezanas | Independent | Not listed | — | No roster |
 University of Sioux FallsDIISioux Falls, SD · Cougars | Northern Sun Intercollegiate Conference | Andy Janssen | 29 | |
 University of South Carolina AikenDIIAiken, SC · Pacers | Peach Belt Conference | Adam Kleman | 31 | |
 University of South Carolina BeaufortDIIBluffton, SC · Sand Sharks | Peach Belt Conference | Gary Boughton | 36 | |
 University of TampaDIITampa, FL · Spartans | Sunshine State Conference | Erin Switalski | 35 | |
 University of Texas at DallasDIIRichardson, TX · Comets | Lone Star Conference | Stephen Shirley | 35 | |
 University of Texas at TylerDIITyler, TX · Patriots | Lone Star Conference | Stefani Webb | 37 | |
 University of Texas Permian BasinDIIOdessa, TX · Falcons | Lone Star Conference | Garrett Kull | 28 | |
 University of West AlabamaDIILivingston, AL · Tigers | Gulf South Conference | Sam Wilson | 24 | |
 University of Wisconsin-ParksideDIIKenosha, WI · Rangers | Great Lakes Intercollegiate Athletic Conference | Brittany Nikolic | 34 | |
 Upper Iowa UniversityDIIFayette, IA · Peacocks | Great Lakes Valley Conference | Patrick Ritter | 30 | |
 Ursuline CollegeDIIPepper Pike, OH · Arrows | Great Midwest Athletic Conference | Jason Kubbins | 30 | |
 Valdosta State UniversityDIIValdosta, GA · Blazers | Gulf South Conference | Stephen Andrew | 32 | |
 Vanguard UniversityDIICosta Mesa, CA · Lions | Pacific West Conference | Emily Reynolds | 32 | |
 Virginia State UniversityDIIEttrick, VA · Trojans | Independent | Frasier Brickhouse, III | 25 | |
 Walsh UniversityDIINorth Canton, OH · Cavaliers | Great Midwest Athletic Conference | Hannah Brown | 24 | |
 Washburn UniversityDIITopeka, KS · Ichabods | Mid-America Intercollegiate Athletics Association | Davy Phillips | 27 | |
