 Shepherd UniversityDIIShepherdstown, WV · Rams | Pennsylvania State Athletic Conference | Braxtin Reddinger2nd season since 2026 | 21 | |
 Shippensburg University of PennsylvaniaDIIShippensburg, PA · Raiders | Pennsylvania State Athletic Conference | Brooke Dellavalle4th season since 2024 | 31 | |
 Shorter UniversityDIIRome, GA · Hawks, Lady Hawks | Conference Carolinas | Sydney Jones2nd season since 2026 | 13 | |
 Slippery Rock University of PennsylvaniaDIISlippery Rock, PA · The Rock | Pennsylvania State Athletic Conference | Taryn Burkholder5th season since 2023 | 26 | |
 Southern Connecticut State UniversityDIINew Haven, CT · Owls | Northeast 10 Conference | Kevin Siedlecki10th season since 2018 | 22 | |
 Southern New Hampshire UniversityDIIManchester, NH · Penmen | Northeast 10 Conference | Carissa MedeirosHead coach since at least 2023 | 31 | |
 Southern Wesleyan UniversityDIICentral, SC · Warriors | Conference Carolinas | Lindsey Schneider11th season since 2017 | 19 | |
 St. Thomas Aquinas CollegeDIISparkill, NY · Spartans | East Coast Conference | Miranda Lopes | 11 | |
 Thomas Jefferson University (Jefferson)DIIPhiladelphia, PA · Rams | Central Atlantic Collegiate Conference | Randy Vite2nd season since 2026 | 18 | |
 Thomas More UniversityDIICrestview Hills, KY · Saints | Great Midwest Athletic Conference | Pat Thatcher6th season since 2022 | 19 | |
 Tiffin UniversityDIITiffin, OH · Dragons | Great Midwest Athletic Conference | Ben Morhac1st season since 2027 | 21 | |
 Tusculum UniversityDIIGreeneville, TN · Pioneers | South Atlantic Conference | Bianca Rojas | 27 | |
 University of Alabama in HuntsvilleDIIHuntsville, AL · Chargers | Gulf South Conference | Akasha Kasper5th season since 2023 | 25 | |
 University of BridgeportDIIBridgeport, CT · Purple Knights | Central Atlantic Collegiate Conference | Not listed | 9 | |
 University of CharlestonDIICharleston, WV · Golden Eagles | Mountain East Conference | Ryland LucianoHead coach since at least 2024 | 41 | |
 University of Colorado Colorado SpringsDIIColorado Springs, CO · Mountain Lions | Rocky Mountain Athletic Conference | Brice Queener15th season since 2013 | 33 | |
 University of FindlayDIIFindlay, OH · Oilers | Great Midwest Athletic Conference | Jordan BlackburneHead coach since at least 2027 | 36 | |
 University of IndianapolisDIIIndianapolis, IN · Greyhounds | Great Lakes Valley Conference | Peyton Romig4th season since 2024 | 30 | |
 University of MontevalloDIIMontevallo, AL · Falcons | Gulf South Conference | Adrianna Piontek7th season since 2021 | 24 | |
 University of Mount OliveDIIMount Olive, NC · Trojans | Conference Carolinas | Katherin StachowskiHead coach since at least 2026 | 25 | |
 University of TampaDIITampa, FL · Spartans | Sunshine State Conference | Kelly GallagherHead coach since at least 2023 | 43 | |
| University of the District of Columbia DIIWashington, DC · Firebirds | East Coast Conference | Zachary Kjeldsen | 10 | |
 Ursuline CollegeDIIPepper Pike, OH · Arrows | Great Midwest Athletic Conference | Iris RayburnHead coach since at least 2024 | 17 | |
| UVA Wise DIIWise, VA · Highland Cavaliers, Cavaliers | South Atlantic Conference | Meghan Dennehy10th season since 2018 | 22 | |
 Virginia State UniversityDIIPetersburg, VA · Trojans | Gulf South Conference | Ashley LawrenceHead coach since at least 2024 | 27 | |
 Walsh UniversityDIINorth Canton, OH · Cavaliers | Great Midwest Athletic Conference | Rachel Black1st season since 2027 | 23 | |
 West Chester UniversityDIIWest Chester, PA · Golden Rams | Pennsylvania State Athletic Conference | Ginny Martino29th season since 1999 | 20 | |
 West Virginia Wesleyan CollegeDIIBuckhannon, WV · Bobcats, Lady Bobcats | Mountain East Conference | Paige Harman | 18 | |
 Westminster UniversityDIISalt Lake City, UT · Griffins | Rocky Mountain Athletic Conference | Ashley TackHead coach since at least 2023 | 31 | |
 Wheeling UniversityDIIWheeling, WV · Cardinals | Mountain East Conference | Maisy LatusHead coach since at least 2025 | 25 | |
 William Jewell CollegeDIILiberty, MO · Cardinals | Great Lakes Valley Conference | Mackenzie PorterHead coach since at least 2026 | 18 | |
 Wilmington UniversityDIINew Castle, DE · Wildcats | Central Atlantic Collegiate Conference | Kevin DayHead coach since at least 2023 | 19 | |
 Wingate UniversityDIIWingate, NC · Bulldogs | South Atlantic Conference | Abby Wiley9th season since 2019 | 31 | |
 Young Harris CollegeDIIYoung Harris, GA · Mountain Lions | Conference Carolinas | Christine Hatton5th season since 2023 | 26 | |
